The Calbiotech DHEA-S Kit is an ELISA for the measurement of DHEA-S in human serum or plasma.
SUMMARY AND EXPLANATION
DHEA-S is a more specific product of the adrenals and measurements of this steroid are widely intended in clinical practice. The clinical importance of plasma assays of DHEA-S is associated with the diagnosis of adrenal hyperplasia and differential diagnosis of hirsutism.
PRINCIPLE OF THE TEST
The DHEA-S ELISA kit is based on the competition principle and the microplate separation. An unknown amount of DHEA-S present in the sample and a fixed amount of DHEA-S conjugated with horse-radish peroxidase compete for the binding sites of a polyclonal DHEA-S antiserum coated onto the wells. After one hour incubation the microtiterplate is washed to stop the competition reaction. Having added the substrate solution the concentration of DHEA-S is inversely proportional to the optical density measured.