Adiponectin ELISA

Posted by admin on May 14th, 2014

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INTENDED USE
The Calbiotech, Inc (CBI) Adiponectin ELISA kit is used for the quantitative measurement of Adiponectin in human serum or plasma.

SKU: AP229G Category:

Description

INTENDED USE
The Calbiotech, Inc (CBI) Adiponectin ELISA kit is used for the quantitative measurement of Adiponectin in human serum or plasma.

SUMMARY AND EXPLANATION
Adiponectin is an adipocyte-secreted hormone, consisting of 244 amino acids with a molecular weight of approximately 30kDa (28-30kDa). It is one of the most abundant proteins in human blood, with circulating concentrations of 0.5-30 μl/mL, which accounts for approximately 0.01% of total plasma protein [1]. Adiponectin concentrations is reversely associated with type 2 diabetes, coronary artery disease and obesity, all together called the metabolic syndrome. Adiponectin decreases blood glucose and free fatty acid serum concentrations and increases insulin sensitivity [2]. Adiponectin has been shown to have anti-inflammatory effects [1]. However, recent studies indicate that Adiponectin may not be present in circulation as monomers or isolated globular forms, but rather in multimeric structures. The studies have shown that the dominant forms of Adiponectin that circulates in human blood are hexamers (LMW) and lager oligomers (HMW) [3,12-14]. The LMW Adiponectin levels does not seem to differ between insulin sensitive- and insulin resistant subjects, nor does LMW Adiponectin differ between men and women. The increased levels of total Adiponectin in insulin sensitive subjects and women were caused by increased amounts of HMW Adiponectin. Both total and HMW Adiponectin showed significant differences between the insulin sensitive- andinsulin resistant subjects.

PRINCIPLE OF THE TEST
The CBI Adiponectin ELISA is a solid phase sandwich enzyme immunoassay. Two monoclonal antibodies are directed against separate antigenic determinants on the Adiponectin molecule. During incubation, Adiponectin in the sample react with anti-Adiponectin antibodies bound to microwell plate wells and anti-Adiponectin antibodies bound to HRP. A simple washing step removes unbound enzyme labeled antibody. The bound conjugate is detected by reaction with 3,3’5,5′-tetramethylbenzidine (TMB). The reaction is stopped by adding acid to give a colorimetric endpoint that is red spectrophotometrically.