Description

Neuron-specific enolase (NSE) is a neuronal form of the glycolytic enzyme enolase, which was first found in extracts of brain tissue, and was later shown to be present in APUD (amine precursor uptake and decarboxylation) cells and neurons of the diffuse neuroendocrine system but not in other peripheral cells. This glycolytic enzyme enolase (2-phospho-D-glycerate hydrolase, EC 4.2.1.11) exists as several dimeric isoenzymes (αα, αβ, αγ, ββ and γγ) composed of three distinct subunits α, β and γ. Three isoenzymes are found in human brain: αα, αγ, and γγ. The γ unit is found either in a homologous γγ- or in a heterologous αγ-isoenzyme and is known as neuron-specific enolase (NSE). NSE is a valuable tumor marker for cancers of neuroendocrine origin, especially for small-cell lung cancer and neuroblastoma.

Principle of the Assay

The Calbiotech NSE ELISA kit is a solid phase direct sandwich ELISA. The standards, samples and controls are added into the selected wells which are pre-coated with anti human NSE monoclonal anybody along with an anti-NSE-HRP conjugated pair match antibody. NSE, in the standards, controls and patient samples binds to anti-NSE antibody in the wells and anti-NSE-HRP conjugated antibody binds to the NSE. The unbound glycolytic enzyme enolase, NSE, is washed off by wash buffer. Upon the addition of the TMB substrate, the intensity of color developed is proportional to the concentration of NSE in the samples. A standard curve is prepared relating color intensity to the concentration of the NSE.

Assay Specifications

Run time: 105min
Dynamic range: 2.5 – 150ng/ml
Sample type: Serum, Plasma, CSF
Sample volume: 25ul