The CBI Mouse/Rat Thryroxine (T4) ELISA Kit is intended for the detection of total T4 in mouse/rat serum or plasma. This procedure provides ultra sensitivity and minimum sample volume (10ul per sample) which are very critical for testing Mouse/Rat samples.
SUMMARY AND EXPLANATION
T4 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T4 is decreased in hypothyroid and is increased in hyperthyroid. The level of T4 is normal in Euthyroid conditions.
PRINCIPLE OF THE TEST
The CBI T4 is a solid phase competitive ELISA. The samples and the diluted T4 enzyme conjugate are added to the wells coated with anti-T4 polyclonal antibody. T4 in the serum competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound T4 and T4 enzyme conjugate is washed off by wash buffer during a wash step. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is generated relating color intensity to the concentration of the T4.