The Calbiotech Mouse/Rat Triiodothyronine (T3) ELISA Kit is intended for the quantitative measurement of Triiodothyronine (T3) in mouse/rat serum or plasma.
SUMMARY AND EXPLANATION
Triiodothyronine (T3) is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T3 is decreased in hypothyroid and is increased in hyperthyroid conditions.
PRINCIPLE OF THE TEST
The Calbiotech Mouse/Rat T3 is a solid phase competitive ELISA. The samples, the working T3 enzyme conjugate, diluted in assay diluent, are added to the wells coated with anti-T3 polyclonal antibody. T3 in the patient’s serum competes with T3 enzyme conjugate for binding sites.
Unbound T3 and T3 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve is prepared relating color intensity to the concentration of the T3.