The Calbiotech (CBI) Lysozyme ELISA Kit is intended for the quantitative measurement of lysozyme in human serum or stool.
Summary and explanation
Lysozyme (muramidase) is an enzyme present in serum, plasma, amniotic fluid, stool, saliva, tears, urine and other biological fluids. Elevated lysozyme levels in urine and serum have been reported in many human disease states, including Crohn’s disease, leukemias (FAB-M4, CMML, CML), tuberculosis, megaloblastic anemias, acute bacterial infections, ulcerative colitis, severe renal insufficiency, pyelonephritis, nephrosis and renal transplant rejection.
PRINCIPLE OF THE TEST
The CBI Lysozyme kit is a solid phase direct ELISA sandwich method. The samples and the working anti-lysozyme enzyme conjugate are added to the wells coated with anti-lysozyme monoclonal antibody. Lysozyme in the patient’s sample is bound to the monoclonal capture antibody and detected with a polyclonal detection antibody. Unbound lysozyme and anti-lysozyme enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of Lysozyme in the samples. A standard curve is generated relating color intensity to the concentration of Lysozyme.