Description

INTENDED USE
The Calbiotech LH ELISA Kit is intended for the quantitative measurement of LH in human serum.

SUMMARY AND EXPLANATION
Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormone-releasing hormone (LH-RH or Gn-RH), that is released by the hypothalamus. LH, also called interstitial cell-stimulating hormone (ICSH) in men, is glycoprotein with a molecular weight of approximately 30,000 Dalton. It is composed of two noncovalently associated dissimilar amino acid chains, alpha and beta. The alpha chain is similar to that found in human thyroid-stimulating hormone (TSH), follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG). LH stimulates ovulation and ovarian steroid production in the female. In the male, LH controls Leydig cell secretion of testosterone. LH is elevated in Luteal phase of menstrual cycle, primary hypogonadism, Gonadotropin-secreting pituitary tumors and menopause. LH is deceased in hypothalamic Gn-RH deficiency, pituitary LH deficiency and ectopic steroid production.

PRINCIPLE OF THE TEST
The LH ELISA kit is a solid phase direct sandwich method. The samples and diluted anti-LH-HRP conjugate are added to the wells coated with Mab to LH beta subunit. LH in the patient’s serum binds to anti-LH MAb on the well and the anti-LH second antibody then binds to LH. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of LH in the samples. A standard curve is prepared relating color intensity to the concentration of the LH.