Description

INTENDED USE OF THE COTININE TEST
The Calbiotech Cotinine Blood Test Direct ELISA Kit is intended for the measurement of Cotinine in serum and urine.

SUMMARY AND EXPLANATION OF THE COTININE TEST
Exposure to tobacco smoke can be detected by measuring nicotine and its metabolites using a Cotinine test. Nicotine has a short half life and is not used as a marker for tobacco smoke exposure. Cotinine, due to its longer half life, has been used in research as a reliable marker for smoking status and smoking cessation studies. The Calbiotech Cotinine Blood Test Direct ELISA Kit is designed for the detection of Cotinine in serum and urine. It can also be adapted for other fluids.

PRINCIPLES OF THE COTININE TEST
The Calbiotech Cotinine Blood Test kit is a solid phase competitive ELISA. The samples and Cotinine enzyme conjugate are added to the wells coated with anti-Cotinine antibody. Cotinine in the samples competes with a Cotinine enzyme (HRP) conjugate for binding sites. Unbound Cotinine and Cotinine enzyme conjugate is washed off by washing step. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of Cotinine in the samples obtained with the Cotinine blood test. A standard curve is prepared relating color intensity to the concentration of the Cotinine.