TheCalbiotech Inc. Alpha 1-Antichymotrypsin (ACT) ELISA Kit is intended for thequantitative measurement of alpha 1-antichymotrypsin in human stool.
PRINCIPLE OF THETEST
The CalbiotechInc. Alpha 1-Antichymotrypsin (ACT)kit is a solid phase direct ELISA sandwich method. The standards, samples andcontrols are added into designated wells, coated with anti-ACT polyclonalantibody, along with the incubation buffer. After a simple washing step, an anti-ACTenzyme conjugate reagent is added into each well. After the excess enzymeconjugate is washed out, the substrate is added into each well. Upon theaddition of the substrate, the intensity of color developed is directly proportionalto the concentration of ACT in the samples. A standard curve is generatedrelating color intensity to the concentration of ACT.