G-6-PDH – Spot Test
METHOD Spot Test, Visual Fluorescence (qualitative)
Glucose + 6-phosphate + NADP (Not Fluorescent) —-> 6-Phophugluconate + NADPH (Fluorescent)
The reaction mixture containing glucose -6-phosphate+NADP (Not Fluorescent) and blood is incubated and, at timed intervals, drops of the mixture are applied to filter paper. The spots are visually inspected under long-wavelength (320-420nm) ultraviolet light. The observed rate of appearance of bright Fluorescence is proportional to the blood G-6-PDH activity.