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G-6-PDH - Dye Reduction

METHOD Dye Reduction, Visual Colour (qualitative)

PRINCIPLE
Glucose-6-phosphate + NADP ——->+ 6-Phosphogluconate + NADPH
NADPH + Blue Day —> NADP – Colourless Dye Complex

The rate at which the colour visually disappears in the reaction mixture is proportional to the G-6-PDH content of red cells.

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